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protein kinase inhibitor (pki) ( 5 – 24 ) amide  (LC Laboratories)

 
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    Structured Review

    LC Laboratories protein kinase inhibitor (pki) ( 5 – 24 ) amide
    Effect of PKA activity on voltage dependence of Ca2+ channel activation. (A) Mean conductance–voltage relationships in control mouse 129 CB3 myotubes (○), myotubes dialyzed intracellularly with PKA (▴), and myocytes dialyzed intracellularly with the <t>PKA</t> <t>inhibitor</t> peptide, <t>PKI</t> (▪). (B) Mean potentiation of Ca2+ channels in 129 CB3 cells (± SEM, n = 20, 9) measured by using the voltage-clamp protocol of Fig. 2.
    Protein Kinase Inhibitor (Pki) ( 5 – 24 ) Amide, supplied by LC Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pki+%285%E2%80%9324%29+amide/pmc00554817-57-0-18?v=LC+Laboratories
    Average 90 stars, based on 1 article reviews
    protein kinase inhibitor (pki) ( 5 – 24 ) amide - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Convergent regulation of skeletal muscle Ca 2+ channels by dystrophin, the actin cytoskeleton, and cAMP-dependent protein kinase"

    Article Title: Convergent regulation of skeletal muscle Ca 2+ channels by dystrophin, the actin cytoskeleton, and cAMP-dependent protein kinase

    Journal:

    doi: 10.1073/pnas.0409695102

    Effect of PKA activity on voltage dependence of Ca2+ channel activation. (A) Mean conductance–voltage relationships in control mouse 129 CB3 myotubes (○), myotubes dialyzed intracellularly with PKA (▴), and myocytes dialyzed intracellularly with the PKA inhibitor peptide, PKI (▪). (B) Mean potentiation of Ca2+ channels in 129 CB3 cells (± SEM, n = 20, 9) measured by using the voltage-clamp protocol of Fig. 2.
    Figure Legend Snippet: Effect of PKA activity on voltage dependence of Ca2+ channel activation. (A) Mean conductance–voltage relationships in control mouse 129 CB3 myotubes (○), myotubes dialyzed intracellularly with PKA (▴), and myocytes dialyzed intracellularly with the PKA inhibitor peptide, PKI (▪). (B) Mean potentiation of Ca2+ channels in 129 CB3 cells (± SEM, n = 20, 9) measured by using the voltage-clamp protocol of Fig. 2.

    Techniques Used: Activity Assay, Activation Assay, Control



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    Effect of PKA activity on voltage dependence of Ca2+ channel activation. (A) Mean conductance–voltage relationships in control mouse 129 CB3 myotubes (○), myotubes dialyzed intracellularly with PKA (▴), and myocytes dialyzed intracellularly with the <t>PKA</t> <t>inhibitor</t> peptide, <t>PKI</t> (▪). (B) Mean potentiation of Ca2+ channels in 129 CB3 cells (± SEM, n = 20, 9) measured by using the voltage-clamp protocol of Fig. 2.
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    Effect of PKA activity on voltage dependence of Ca2+ channel activation. (A) Mean conductance–voltage relationships in control mouse 129 CB3 myotubes (○), myotubes dialyzed intracellularly with PKA (▴), and myocytes dialyzed intracellularly with the <t>PKA</t> <t>inhibitor</t> peptide, <t>PKI</t> (▪). (B) Mean potentiation of Ca2+ channels in 129 CB3 cells (± SEM, n = 20, 9) measured by using the voltage-clamp protocol of Fig. 2.
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    <t> PKI </t> inhibition of <t> cAMP-stimulated </t> whole-cell Cl conductance
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    Image Search Results


    Effect of PKA activity on voltage dependence of Ca2+ channel activation. (A) Mean conductance–voltage relationships in control mouse 129 CB3 myotubes (○), myotubes dialyzed intracellularly with PKA (▴), and myocytes dialyzed intracellularly with the PKA inhibitor peptide, PKI (▪). (B) Mean potentiation of Ca2+ channels in 129 CB3 cells (± SEM, n = 20, 9) measured by using the voltage-clamp protocol of Fig. 2.

    Journal:

    Article Title: Convergent regulation of skeletal muscle Ca 2+ channels by dystrophin, the actin cytoskeleton, and cAMP-dependent protein kinase

    doi: 10.1073/pnas.0409695102

    Figure Lengend Snippet: Effect of PKA activity on voltage dependence of Ca2+ channel activation. (A) Mean conductance–voltage relationships in control mouse 129 CB3 myotubes (○), myotubes dialyzed intracellularly with PKA (▴), and myocytes dialyzed intracellularly with the PKA inhibitor peptide, PKI (▪). (B) Mean potentiation of Ca2+ channels in 129 CB3 cells (± SEM, n = 20, 9) measured by using the voltage-clamp protocol of Fig. 2.

    Article Snippet: Protein kinase inhibitor (PKI) ( 5 – 24 ) amide, calyculin A, and cytochalasin D were obtained from LC Laboratories (Woburn, MA), and phalloidin and protein phosphatase inhibitor 2 were obtained from Calbiochem.

    Techniques: Activity Assay, Activation Assay, Control

    PKI inhibition of cAMP-stimulated whole-cell Cl conductance

    Journal:

    Article Title: Cardiac G sα overexpression enhances L-type calcium channels through an adenylyl cyclase independent pathway

    doi:

    Figure Lengend Snippet: PKI inhibition of cAMP-stimulated whole-cell Cl conductance

    Article Snippet: The cAMP-dependent protein kinase inhibitor (PKI 5–24 Amide; Peninsula Laboratories) was used at a final concentration of 5.6 μM.

    Techniques: Inhibition